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provided gbm cell lines  (ATCC)


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    ATCC provided gbm cell lines
    ATF3 is involved in the ERS of <t>GBM</t> cells treated with LIFU. ( A ) Annexin-V/PI <t>bound</t> <t>U251</t> or <t>LN229</t> cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .
    Provided Gbm Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/provided+gbm+cell+lines/pmc13162950-50-5-1?v=ATCC
    Average 99 stars, based on 1947 article reviews
    provided gbm cell lines - by Bioz Stars, 2026-06
    99/100 stars

    Images

    1) Product Images from "Piezo1-ATF3-PPP1r15a Axis Transduces Mechanical Stress into Apoptosis in Glioma Under Low-Intensity Focused Ultrasound"

    Article Title: Piezo1-ATF3-PPP1r15a Axis Transduces Mechanical Stress into Apoptosis in Glioma Under Low-Intensity Focused Ultrasound

    Journal: Cancers

    doi: 10.3390/cancers18091445

    ATF3 is involved in the ERS of GBM cells treated with LIFU. ( A ) Annexin-V/PI bound U251 or LN229 cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .
    Figure Legend Snippet: ATF3 is involved in the ERS of GBM cells treated with LIFU. ( A ) Annexin-V/PI bound U251 or LN229 cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .

    Techniques Used: Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Staining, Control



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    provided gbm cell lines  (ATCC)
    99
    ATCC provided gbm cell lines
    ATF3 is involved in the ERS of <t>GBM</t> cells treated with LIFU. ( A ) Annexin-V/PI <t>bound</t> <t>U251</t> or <t>LN229</t> cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .
    Provided Gbm Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/provided+gbm+cell+lines/pmc13162950-50-5-1?v=ATCC
    Average 99 stars, based on 1 article reviews
    provided gbm cell lines - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier

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    ATF3 is involved in the ERS of GBM cells treated with LIFU. ( A ) Annexin-V/PI bound U251 or LN229 cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .

    Journal: Cancers

    Article Title: Piezo1-ATF3-PPP1r15a Axis Transduces Mechanical Stress into Apoptosis in Glioma Under Low-Intensity Focused Ultrasound

    doi: 10.3390/cancers18091445

    Figure Lengend Snippet: ATF3 is involved in the ERS of GBM cells treated with LIFU. ( A ) Annexin-V/PI bound U251 or LN229 cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .

    Article Snippet: The American Type Culture Collection provided GBM cell lines (U251 and LN229) and the 293T cell line.

    Techniques: Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Staining, Control